Comparison of the biological behavior of hormone-sensitive prostate cancer cell line 22Rv1, before and after the implantation of xenograft in immunodeficient mouse models (Bachelor thesis)

Παπαδάκη, Ελένη

Cancer is not just a mass of malignant cells, but a complicated system which includes healthy cells, blood vessels and other components and has its own special features (temperature, pH). The environment, despite the cancer cells, is generally called "microenvironment of tumor" and it is very interesting to study how the cancer cells are affected by it. The purpose of this thesis is to compare the behavior of human prostate cancer cells 22Rv1 before and after the xenograft implantation in a SCID mouse. Initially, 22Rv1 cells will be implanted and when the tumor reaches 0.7 x 0.7 cm, the animal will be sacrificed and the tumor removed. With an appropriate protocol, the tumor will be solved and the 22Rv1p1 cell line will be obtained. Next, the rate of proliferation of the two cell lines will be compared by growth rate monitoring (AlamarBlue® method) and confocal microscopy, studying the Ki67 proliferation marker and calculating the percentage of mitoses. Subsequently, the basal expression of the full-length androgen receptor (AR) and the ARV7 splice variant in the two cell lines will be compared with the Western immunoblotting technique. Finally, the migration ability of 22Rv1 and 22Rv1p1 will be studied with the ScratchAssay method.
Institution and School/Department of submitter: Δημοκρίτειο Πανεπιστήμιο Θράκης. Σχολή Επιστημών Υγείας. Τμήμα Μοριακής Βιολογίας και Γενετικής
Subject classification: Prostate--Cancer
Keywords: Prostate,Cancer,Xenograft,mice,Προστάτης,Καρκίνος,Αλλομόσχευμα,Μύες

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