Η επίδραση των πρωτεϊνών δέσμευσης RNA (RBPs) στο σηματοδοτικό μονοπάτι mTOR καθώς και στην βιωσιμότητα των κυττάρων (Bachelor thesis)

Δημοπούλου, Αναστασία/ Dimopoulou, Anastasia

The mTOR pathway is an intracellular signaling pathway, with cell cycle regulation as its main role. It is involved in several processes including protein synthesis and regulation of autophagy and its upstream activation is mediated by growth factors, ATP and oxygen levels, and DNA damage. The aim of this thesis is to study the effect of RNA binding proteins (RBPs) on the mTOR pathway, as well as on the viability of cells when overexpression occurs. Proteins that bind to mRNAs have an important role in post-transcriptional regulation. RBPs are involved in post-transcriptional modifications such as alternative splicing of the mRNA precursor, extraction of the mRNA from the nucleus to the cytoplasm, polyadenylation, stabilization or degradation, and translational regulation processes. In the present study, the SHSY5Y cell line was used, where the RBPs TIA1, HuR, HuB, HuC, HuD, TDP43, TDP43-ΔNLS, FUS, Fox2, Fox3 and proteins REDD1, DYRK1A and SNCA were overexpressed. Western immunoblotting was employed to detect expression of both phosphorylated and non-phosphorylated proteins of mTOR pathway, as well as the ERK1/2 protein. Western immunoblotting was, also, employed to detect expression of puromycin, for the evaluation of protein synthesis. Cell viability was, further, assessed by the ATP assay The results indicated that the RBPs and REDD1, Dyrk1A and SNCA proteins overexpression directly or indirectly regulate the mTOR pathway, of which REDD1 and HuR affect both protein synthesis and cell viability.
Institution and School/Department of submitter: Δημοκρίτειο Πανεπιστήμιο Θράκης. Σχολή Επιστημών Υγείας. Τμήμα Μοριακής Βιολογίας και Γενετικής
Subject classification: Carrier proteins
Keywords: RNA binding proteins,mTOR pathway,Cell viability,Πρωτεΐνες δέσμευσης RNA,Μονοπάτι mTOR,Βιωσιμότητα κυττάρων
URI: https://repo.lib.duth.gr/jspui/handle/123456789/17192
http://dx.doi.org/10.26257/heal.duth.15926
Appears in Collections:ΤΜΗΜΑ ΜΟΡΙΑΚΗΣ ΒΙΟΛΟΓΙΑΣ & ΓΕΝΕΤΙΚΗΣ

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http://dx.doi.org/10.26257/heal.duth.15926
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