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Λεμφοκυτταρικοί δείκτες ανοσολογικής αντι-νεοπλασματικής ανταπόκρισης κατά τη συγκαλλιέργεια λεμφοκυττάρων με καρκινικά κύτταρα πνεύμονα (Bachelor thesis)

Σιώπη, Ζωή

The purpose of this research is to study the immune profile of peripheral blood lymphocytes of patients during co-culture of lymphocytes with lung cancer cells. Lymphocytes were isolated from peripheral blood of patients by centrifugation in Ficoll-Hypaque. The separation is based on the fact that the lymphocytes have a lower density than the red blood cells and the granulocytes. We cultured lymphocytes, cancer cell line of lung A549 and co-culture of the above cells. By Western blotting technique we detected the CD4 protein expression (potential regulatory lymphocytes suppress the anti-neoplastic immune response), and PD-1 (immune checkpoint receptor suppressing the action of cytotoxic lymphocytes after PD-L1-binding). With these experiments we found that lymphocyte anti-neoplastic potential is increased after their contact with cancer cells.