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Population genetic survey in Pinus nigra (Bachelor thesis)

Γιαννακόπουλος, Νικόλαος - Ευάγγελος/ Giannakopoulos, Nikolaos - Evangelos

The black pine (Pinus nigra) is a coniferous tree of the Pinaceae family that is mainly located in the Mediterranean basin. Due to its extensive geographical distribution in a wide range of habitats, Pinus nigra exhibits great genetic diversity, while also presenting high financial interest in the furniture and wood industry. The genetic basis of its adaptation to natural habitats is an interesting research field, in view of climate change combatment. In this research, the study of the evolutionary differences of black pine populations from four regions of Macedonia, Athos, Sithonia, Thasos and Perama, was carried out, with the use of nuclear microsatellites. Nuclear DNA was isolated from the pine needles of Pinus nigra nigra, of which nine microsatellite regions were amplified using multiplex PCR. Electrophoresis of the amplified sequences was followed, to find specific band patterns, and after fragment analysis was conducted, the results were interpreted with the use of the Geneious Prime program.